Effect of Exogenously Applied 24-Epibrassinolide and Brassinazole on Xylogenesis and Microdistribution of Cell Wall Polymers in Leucaena leucocephala (Lam) De Wit

Abstract Plant growth regulators play a key role in cell wall structure and chemistry of woody plants. Understanding these regulatory signals is important advanced research on wood quality improvement trees. The present study aimed to investigate the influence exogenous application 24-epibrassinolide (EBR) brassinosteroid inhibitor, brassinazole (BRZ) formation spatial distribution polymers xylem tissue Leucaena leucocephala using light immuno electron microscopy methods. Brassinazole caused decrease cambial activity, differentiation, length width fibres, vessel element radial extent suggesting inhibition has concomitant impact elongation, expansion secondary deposition. Histochemical studies treated plants showed an increase syringyl lignin content walls. Fluorescence transmission revealed inhomogenous pattern corners middle lamellae region BRZ Immunolocalization LM10 LM 11 antibodies have shown drastic change micro-distribution less substituted highly xylans fibres with EBR . In conclusion, demonstrates plant development through regulating xylogenesis higher